FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9015512
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG6100.017.334633
CCCGTAT7000.015.8059361
CCGTATC7850.013.7568212
ATACGGC3400.013.2341965
TCGTATG6200.013.06372143
GTATCGA7900.012.5306064
CCGTCAT9400.011.7703781
CTCGTAT8050.011.45893142
CGTATGC7400.011.24931644
CGATAAC6050.011.1966491
GTACGAT7500.010.49912925
GCGCTAA7400.010.3372142
ACGCTAC7750.010.1604485
GACGCTA6950.010.0351384
TATATCG7400.010.03295135
TCAATCG8250.09.8173683
CCGGGAT13000.09.7267291
CGCTAAG8400.09.64227143
CGACTAA4904.9112714E-119.64205728
ACGATCT8700.09.568171537
Produced by FastQC (version 0.11.5)