FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001249
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG21650.020.88783843
CTCGTAT22750.020.07538642
CGTATGC23050.019.91198544
GATCGGA26650.016.9455911
ACACGTC30100.015.47215213
GTATCGA14300.014.3170074
CCCGTAT14600.013.9191781
TCTCGTA33800.013.84509441
GTATGCC36300.013.20165345
CACGTCT35850.012.80229714
CGTATCG18300.012.2940733
CACACGT39600.012.21490712
CATCTCG38500.011.97953639
CACTTAG39800.011.92741231
TCGGAAG40800.011.6350743
ACGTCTG40400.011.52751915
ACTTAGG42100.011.00859632
ATCTCGT43000.010.83050640
CTTAGGC43550.010.79704733
CGCGTAT2107.89872E-510.752381
Produced by FastQC (version 0.11.5)