FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026954
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10200.023.38056843
CGTATGC10800.022.28996544
CTCGTAT12350.019.31026642
AAGCGAC20550.017.07898920
TCGCTAT22700.016.05604428
CGCTATG22600.015.92798829
GAACGCT23300.015.06322835
AAAAGCG24150.014.99885718
CCGACAT24700.014.9896041
GTCGCTA24950.014.78845127
ATCGAAG30900.014.7804256
CGCCAAT16750.014.37203833
TCGAAGG32900.014.3606067
AGTCACG16400.014.26720328
TATCTCG16950.014.20245639
GCGGTAT11850.014.0979731
CGACATC27450.014.0972842
GTATCGA6200.013.4263944
CGAAGGA27700.013.4015058
GATCGGA17650.013.3024591
Produced by FastQC (version 0.11.5)