FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19978827
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26250.026.14088643
CTCGTAT27000.025.66473242
CGTATGC27150.025.10860444
TCTCGTA32700.021.12226141
GATCGGA34000.020.1839181
ACACGTC37850.018.48605513
ATCTCGT41300.016.61497140
ACGTCTG42650.016.35281215
GTATGCC45450.015.642385545
CACGTCT45800.015.62109214
ATCGGAA44450.015.4881462
TCGGAAG48400.014.5960063
CATCTCG49150.014.00708239
CACACGT51250.013.95992312
CGTCTGA58750.012.56075516
CGGAAGA60050.012.1389684
GCACACG62900.011.76779311
CCCGTAT13250.010.3926141
AATCGGG17100.010.2623845
CGCGTAA5500.010.2609631
Produced by FastQC (version 0.11.5)