FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7837979
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9500.024.39291243
CTCGTAT10050.023.50570742
CGTATGC10000.023.1732744
GATCGGA10950.022.6772861
AATCTCG11350.020.615239
TCTCGTA12600.018.92715841
ACACGTC14400.017.49869313
ATCTCGT13550.017.43412240
ACGTCTG14800.017.02575515
ATCGGAA14700.016.8354762
CACGTCT16700.015.62757514
CGTATCG4800.015.4675943
TCGGAAG16750.015.1779713
CACACGT19650.013.39594612
GTATCGA5250.013.2847224
CGACTAA6900.013.04250328
GTATGCC19400.012.29289545
CGGAAGA21300.012.2526054
CCCGTAT6150.012.112991
GCACACG22450.011.52475211
Produced by FastQC (version 0.11.5)