FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20015721
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18250.026.25831843
CTCGTAT20150.023.6706942
CGTATGC23350.020.52309844
GTCACGA25150.019.2331629
AGTCACG25650.019.0336728
TCTCGTA26700.017.86383841
ACGATCA28000.017.275532
GATCGGA28700.016.7532331
TCACGAT29450.016.34852830
CGTATCG15150.015.7414013
ACGTCTG33200.014.70526615
ACACGTC32900.014.56582113
CACGATC34650.014.0898631
CGATCAG35500.014.06937433
ATCTCGT34700.014.00472140
CCCGTAT18100.013.5940491
CACGTCT35800.013.57444114
ATCGGAA35250.013.4032572
GTATCGA16900.013.3126174
CCGTATC21000.012.320512
Produced by FastQC (version 0.11.5)