FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25314222
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14450.016.3482143
CGTATGC18850.013.12895244
CGCGTAA10250.012.334411
CTCGTAT19650.012.25095642
TATCTCG26150.010.15217439
GCGGTAT16200.09.8945851
GTATCGA14850.09.8477194
CCGGAAT30000.09.7078421
CGTATCG17500.09.5135453
AATCGGG23100.09.3499225
CCGACAT29250.09.1849191
GAATCGA20950.09.1281684
GATCGGA26200.08.9616151
CCCGTAT18900.08.9588791
AATCGAA26400.08.9481685
CGGAAAT29300.08.9380871
CGAATCG7050.08.9354753
ATCGAAC21750.08.7924196
GTCGCTA14800.08.66486627
CGCGGAT14150.08.4561681
Produced by FastQC (version 0.11.5)