FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24046086
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34300.020.13695343
CTCGTAT37250.018.30062342
CGTATGC37950.018.2594844
AGATCGG31500.016.338871
GATCGGA41450.016.0110761
ACACGTC45900.014.85190413
GTATGCC47800.014.54387445
CACGTCT52400.013.13839614
AAGCGAC33800.012.78009420
TCTCGTA57000.012.63061941
CGCTATG35450.012.24879329
CACACGT58650.011.96847412
CCGACAT43900.011.723791
GAACGCT37500.011.63918835
TCGCTAT36800.011.61596428
ATCGAAG52900.011.3986
TCGGAAG58800.011.3639333
GCGGTAT17750.011.1913381
GTAATCT62650.011.13243437
GTCGCTA39100.011.04775327
Produced by FastQC (version 0.11.5)