FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31570787
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG605200.1916962032020298TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80200.038.15199745
CTCGTAT97100.031.233744
GATCGGA102600.028.1537281
ACACGTC114550.026.78995713
ATCTCGT117800.025.76436442
ACGTCTG120900.025.58757815
CACGTCT128150.024.1224214
AATCTCG127000.023.98654641
CACACGT129200.023.96120612
ATCGGAA120950.023.940112
TCGGAAG123150.023.6220113
TCTCGTA135100.022.58172443
AGTCAAC142050.021.93633534
GCACACG146850.021.55623411
CGGAAGA150750.020.864244
CGTCTGA159850.019.549816
CAGTCAA161100.019.42616733
GTCAACA160600.019.30452735
AACAATC159950.019.21406438
ACAATCT175200.017.54160539
Produced by FastQC (version 0.11.5)