FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17940611
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28950.022.45938743
CGTATGC30650.021.28708544
CTCGTAT31300.020.7012642
GATCGGA35800.018.4775331
ACCGATG36500.018.4301732
TCTCGTA37250.017.69660641
ATCTCGT38750.017.06963540
ACACGTC39350.016.86653713
CGATGTA40800.016.0466334
TATCTCG42100.015.65792439
TCACCGA43800.015.35843330
GTCACCG43800.015.30706729
CACCGAT44100.015.25399631
CGTATCG16300.015.1828653
GTATCGA16000.015.04574
CCGATGT45300.014.89958333
ACGTCTG44550.014.79682515
CCCGTAT16500.014.7774391
ATCGGAA45250.014.6176442
CACGTCT46700.014.35648214
Produced by FastQC (version 0.11.5)