FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10394499
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG109820.10565203767877605TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15700.035.25234245
CTCGTAT17250.031.9543144
ACGTCCG20900.027.23508832
GATCGGA19500.027.1968461
TCTCGTA22200.024.93070643
AGTCACG22650.024.8327228
ATCGGAA21500.024.6960852
ATCTCGT23100.024.2515742
GTCACGT24800.023.31503329
CGTCCGC24950.023.26503633
CGCACAT24500.023.14125637
ACACGTC24400.022.95947613
GTCCGCA25000.022.8585334
ACGTCTG26300.021.47189915
CACGTCT27050.021.04290614
TCACGTC27700.020.71166230
TCCGCAC28900.020.31876235
TCGGAAG27800.019.099493
CCGCACA30300.018.93442536
CACACGT30100.018.6116712
Produced by FastQC (version 0.11.5)