FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7380566
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9000.030.99785645
TATCTCG10350.026.95465941
CTCGTAT10550.026.65692344
GATCGGA13200.021.3718011
GATATAT14950.019.26288237
ATATATC15250.019.17938
ACACGTC15650.018.25752413
ATATCTC16550.017.80843740
TATATCT16950.017.3881839
ACTGATA16200.017.35991134
CACGTCT16900.017.04024314
CGTCTGA17000.016.80766316
CTGATAT17550.016.4091235
TCGGAAG17400.016.1626763
CACACGT18500.015.44487812
TGATATA21050.014.642693536
GTCACAC19750.014.23952329
TCGCTAT10150.013.96455128
CCGGTAT6400.013.7527541
GCACACG22350.013.58966411
Produced by FastQC (version 0.11.5)