FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22428779
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG379820.16934492956571556TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50650.036.201745
CTCGTAT60650.030.1214644
TATCTCG63950.028.70783241
GTCACGA64100.028.60555529
AGTCACG67750.027.19727528
CACGAGT67500.027.03141831
ACGAGTG68350.026.72817432
GATCGGA71450.026.1579341
TCACGAG71550.025.94155930
GGATATC73000.025.39540538
ACACGTC74650.025.13547113
GATATCT75850.024.47085439
CGAGTGG78750.023.16978633
CACGTCT80850.023.06881514
TCGGAAG83000.022.7423153
CACACGT85250.022.08929412
ATATCTC84950.021.82300840
TCTCGTA89150.021.55198743
GTGGATA89150.020.56780836
TGGATAT94250.019.52642437
Produced by FastQC (version 0.11.5)