FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20888752
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG605990.29010349684844744TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT235930.11294595292241488TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG73800.039.90580445
CGTACGT98950.029.9676334
TACGTAA98800.029.8992736
CGTAATC98850.029.54274438
ACGTAAT100700.029.26810637
CTCGTAT101050.028.9663544
GTACGTA104900.028.2249535
ACCGTAC106150.027.8501932
ACACGTC108650.027.1886613
GATCGGA113750.026.7640761
GTCACCG115400.025.57883829
CACGTCT116050.025.51311914
CACCGTA117600.025.23424131
CACACGT120150.024.8672112
GTAATCT118600.024.79383539
AGATCGG47400.024.7580641
TCGGAAG122800.024.7336483
TGATACG14400.024.529573
ATACGGC14900.024.3104175
TCTCGTA125850.023.93757643
Produced by FastQC (version 0.11.5)