FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6685908
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC92640.13856008787437696TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15300.029.40952743
CGTATGC16100.027.94818344
CTCGTAT17150.026.49944142
GATCGGA18650.025.0571121
TCTCGTA18950.024.10106841
ACACGTC19550.023.59155513
GTATGCC20000.023.06074545
ATCTCGT20550.022.11510540
AATCTCG20800.021.95746239
ACGTCTG21500.021.45185515
ATCGGAA22750.020.4709682
CACGTCT22700.020.31783714
TCGGAAG24250.019.2047233
CACACGT24700.018.49049412
GTCACAC28250.016.88366929
TTGAATC27900.016.77291736
GAATCTC28200.016.75404438
CGTCTGA28400.016.55683716
CGGAAGA28450.016.369584
GCACACG29150.016.2852111
Produced by FastQC (version 0.11.5)