FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29261004
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42050.020.88214943
CGTATGC45500.019.3482644
CTCGTAT46500.018.6407942
ACACGTC52200.017.02205313
CGCGTAA39000.016.8054961
GATCGGA48150.016.6056181
GTCGCTA41750.016.43344927
AGATCGG34100.015.323451
CGCTATG45750.014.94747529
CCGACAT50850.014.8823421
CACGTCT61950.014.48828414
AAGCGAC48600.014.11721120
CACACGT65500.013.70304112
GCGTAAC48800.013.6937662
AAAAGCG52450.013.42406918
TCGGAAG62100.013.2972613
TCGAAGG73750.013.1463457
CGACATC58300.013.0833032
GTATCGA20150.012.9529894
ATCGAAG77700.012.7097726
Produced by FastQC (version 0.11.5)