FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11314822
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15100.025.79710243
CGTATGC16550.023.53693244
CTCGTAT16550.023.53526742
ACACGTC20600.019.98320613
GATCGGA18950.019.8502771
TCTCGTA19850.019.84787441
AATCTCG19900.019.79257839
ACGTCTG22450.017.93568415
CACGTCT23800.017.29638714
ATCGGAA21750.016.965462
ATCTCGT24950.015.78820940
CACACGT27100.015.60521612
GTATGCC26650.015.39669945
TCGGAAG27850.013.5726653
CGTCTGA30050.013.47439616
GCACACG34400.012.35903611
TTGAATC35200.011.76220836
CGGAAGA34400.011.5768944
GTCACAC35550.011.45307629
GAATCTC35550.011.1406838
Produced by FastQC (version 0.11.5)