FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16630866
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG279410.16800688551035164TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38400.035.62248645
CTCGTAT41700.032.4257744
GATCGGA47600.029.2557371
TCTCGTA46550.029.19235643
CGTCCGC48850.028.55479233
AGTCACG48900.028.20353128
GTCACGT50750.027.3527529
ACGTCCG51100.027.29748732
CGCACAT51050.027.28015337
ATCTCGT50600.027.03366742
GTCCGCA52050.026.66958834
ACACGTC52600.026.21963313
ACGTCTG52850.026.05303615
ATCGGAA54400.025.5588572
CCGCACA54750.025.5187536
TCCGCAC56800.024.55812535
CACGTCT57150.024.21089214
TCACGTC57500.024.18090230
TCGGAAG61300.023.1957513
CATCTCG60800.022.90545541
Produced by FastQC (version 0.11.5)