FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126385
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13900.029.9658243
CTCGTAT14550.029.09031942
CGTATGC15100.027.43532844
GATCGGA15850.023.8737811
GTCACGA17600.023.51742429
AGTCACG18200.023.48371728
ACGATCA18250.022.67981732
TCTCGTA18950.022.21533241
TCACGAT19600.021.34722930
CACGATC20150.020.54127731
ACACGTC20700.020.10416413
ATCTCGT20550.020.04468240
ACGTCTG21500.019.66998515
CGATCAG21150.019.4650133
ATCGGAA19400.019.2517832
CACGTCT24150.017.32528714
CACACGT25450.016.5286912
TCGGAAG23900.015.9093883
GTATGCC28650.015.26743445
CGTATCG5550.014.5939933
Produced by FastQC (version 0.11.5)