FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17875111
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG438350.24522924640859572TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48800.039.04941645
CTCGTAT54650.034.78703744
GATCGGA63800.030.3203851
TCTCGTA63800.029.86851743
CCGATCT64000.029.84548439
CGTCCCG65450.029.32177735
ATCTCGT65700.029.0047442
GTCCCGA66050.028.91916836
CCCGATC66050.028.91916838
ACACGTC68000.028.5530713
ATCGGAA69650.027.8120482
ACCCGTC69400.027.75014332
ACGTCTG71400.027.44548415
TCCCGAT71400.026.87829837
CACGTCT74300.026.4045414
TCGGAAG75000.025.9181143
CACCCGT77300.025.05962631
CACACGT79750.024.45903612
GATCTCG80200.023.84489841
TCACCCG83000.023.41998530
Produced by FastQC (version 0.11.5)