FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13221816
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC159090.12032386473991166TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC140980.10662680527395027TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG27100.025.0788761
CGCGTAA25600.020.638911
TCGTATG33850.020.27164644
CTCGTAT34700.019.58056843
CGTATGC34850.019.56084845
GTATGCC35500.019.07594145
AAGCGAC29150.017.52004220
CGCTATG29000.017.37792429
CCGACAT31150.017.179131
GATCGGA40300.017.138832
GCGTAAC31350.017.0082112
ACACGTC41050.016.66127613
AAAAGCG31400.016.40792718
GTCGCTA30450.016.4026327
TCGCTAT32850.015.34124128
CGTAACT35500.015.1466783
CGACATC35700.015.1248432
ATCGAAG40350.015.1103186
GAACGCT33900.014.93243635
CACGTCT46400.014.88566615
Produced by FastQC (version 0.11.5)