FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13176266
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG17400.029.60983343
CGTATGC18300.028.5224444
CTCGTAT19150.026.5515242
GATCGGA23050.022.524631
TCACGAT23200.022.40149735
TCTCGTA23150.021.96378541
ACGATCT24450.020.88815137
ACACGTC25200.020.71272313
ATCTCGT25350.020.23515940
ATCGGAA25850.020.1048642
ACGTCTG26600.019.45341715
CACGATC27400.018.88557836
CATCACG29650.017.83184233
CACGTCT29950.017.57797214
ATCACGA30750.017.48661834
TCGGAAG32450.016.2930683
CACACGT32250.016.25458712
GTATGCC32750.016.21251745
GATCTCG36000.014.68639239
CGTATCG8650.014.0451823
Produced by FastQC (version 0.11.5)