FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791267
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA20200.013.81077427
CCGACAT22600.013.7875661
AAGCGAC20850.013.48812820
GCGGTAT9250.013.4257321
TCGCTAT22050.012.85611328
CGCTATG22250.012.74055329
CGACATC28650.012.4073822
TCGTATG11050.012.01261143
TCGAAGG35600.012.0074647
ATCGAAG35950.011.8905636
AAAAGCG24250.011.87533718
CGTATGC11000.011.86268544
CGGTCTA16400.011.52346644
GCGCTAA11150.011.5013242
GAACGCT25650.011.31488235
ACATCGA30100.011.2117264
CGACAAC9800.011.0594141
GACATCG33450.010.9632473
CGAAGGA29350.010.7316788
ATCGGTA13050.010.34399818
Produced by FastQC (version 0.11.5)