FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15151917
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14450.031.29542245
CTCGTAT17650.025.74893644
AGTCACG20350.022.77484328
TATCTCG20900.021.85256441
GTCACGT22750.020.27386329
ACACGTC25300.018.31889713
GATCGGA23000.018.1493431
CCGACAT31400.015.8810661
TCTCGTA32950.015.77281443
AAGCGAC29800.015.32612620
CACGTCT30300.015.22172414
GTCGCTA32000.014.97553127
TCACGTG30900.014.92655130
CACACGT32150.014.69572212
GCCTTAT33000.014.38542137
CGCTATG32400.014.30494829
CGACATC35350.014.1927992
TCGAAGG42350.014.0250087
AAAAGCG33700.013.88628418
TCGCTAT33800.013.84520128
Produced by FastQC (version 0.11.5)