FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791114
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18050.030.16400545
CTCGTAT19900.026.90758344
TATCTCG22400.023.90450541
TCTCGTA25350.021.65522643
GATCGGA26350.020.473061
AGTCACG28400.019.40879428
GTCACGT28850.019.0281429
ACACGTC30450.018.02824213
ATCTCGT30950.017.5916142
TCACGTG33350.016.66300830
CACGTCT35350.015.52927714
ACGTGGC37150.015.01914532
GGCCTTA37750.014.95922536
ACGTCTG37050.014.93818115
TCGGAAG38600.014.6880473
ATCGGAA37350.014.5170132
CACACGT38350.014.49046912
CCCGTAT12100.014.3638481
GCCTTAT39650.014.24238837
GTATCGA11350.013.08274654
Produced by FastQC (version 0.11.5)