FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15393841
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG17950.030.45739445
CTCGTAT26150.020.99273744
GATCGGA30100.018.1478021
AGTCACG30450.017.88047228
CGTTTCG31100.017.72379133
GTCACGT31150.017.69534129
ACGTTTC32250.016.88249432
ACACGTC33050.016.81420713
TCTCGTA36000.016.81127743
CGCGTAA16950.015.8471951
CACGTTT36400.014.95770331
CACGTCT37650.014.52085614
CCCGTAT12250.014.0040181
TCGGAAG39900.013.9839463
GTATCGA12450.013.9146514
CACACGT40700.013.819633512
ATCGAAG35350.013.6199436
CCGACAT25500.013.5433591
TTTCGGA41700.013.380321535
GTCGCTA19800.013.29448827
Produced by FastQC (version 0.11.5)