FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703792
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7950.016.41393745
CTCGTAT8850.014.74472344
CCCGTAT10250.014.5329131
GTATCGA10450.013.5636374
ATTAGCG2205.522452E-813.2945176
CGACTAA11250.012.99908328
GTCGCTA9900.012.49911827
ATCGAAG25250.012.2961636
CGTATCG13250.012.2255533
TCGAAGG24800.011.7935237
ACGCGCG7100.011.72452520
CGCGTTA5600.011.65096432
TCGATTG10100.011.13782820
TCAATCG12750.010.7639473
CCGTATC16350.010.5955832
CGACCGT9350.010.58748932
AAGCGAC11400.010.45978820
TGAAACG13000.010.383882535
GACGCTA10900.010.3203734
CCGACAT13150.010.2981241
Produced by FastQC (version 0.11.5)