FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30694135
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24750.015.6446343
CGTATGC28150.013.51513444
CTCGTAT28400.013.47516742
GTATCGA21100.012.5842374
TATCTCG34300.011.61304439
CGTATCG20350.011.3896413
CCCGTAT21400.011.2564971
GATCGGA30800.010.8179331
ACACGTC38650.09.95185713
TCTCGTA40800.09.87522241
CGCGTAT3309.861596E-79.5509671
GCGGTAT18300.09.349661
CGCGAAC6800.09.2700581
ATCTCGT45050.09.0426740
GCGGAAT31350.08.9764741
CGCGGAT17950.08.9048621
CCGGGAT46200.08.8200261
TAGCTTA45300.08.59145934
ACTAGCT45600.08.583055532
CCGTATC31850.08.5489572
Produced by FastQC (version 0.11.5)