FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005740931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124400777
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTG5289200.4251741932447897No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAA4509710.3625146167696364No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC3251800.26139708114524074No Hit
GGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAG2786930.224028343488562No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2389430.19207516686169895No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC2358390.18958000559755345No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG2337990.18794014445745785No Hit
GTTTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTC2314350.18603983478334704No Hit
AGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGA2266540.18219661119962297No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTC2190380.176074462943266No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2131800.17136548913999147No Hit
CATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAG2034920.1635777564315374No Hit
AATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCATA1902640.15294438233291743No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1887660.1517402097898472No Hit
GTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT1808240.145356005292475No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1745770.1403343324776822No Hit
GGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATC1713990.13777968605453325No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1710300.13748306411301595No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1645930.13230865913321427No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1595440.1282500028114776No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1547040.12435935187125077No Hit
GCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAGC1532860.12321948760818432No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1531600.12311820206717841No Hit
GTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATC1525620.12263749767415037No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATG1445730.11621551206227594No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1413790.11364800398312624No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA1382920.11116650822848156No Hit
GAGCTCTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTT1370500.11016812218142336No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1368740.11002664396541512No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1333580.10720029505925033No Hit
ATCAAAGGTCCCAGGTTCCAGGTTGTCCCTAAGAGCCTGAACGATAAGAA1330930.1069872738817379No Hit
TGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGT1268690.10198408969744618No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA1263290.10155000880742086No Hit
AACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGAT1260870.10135547626040954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)