FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005740945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140507135
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTG3285590.2338379470907296No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAA3071510.21860171015514623No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC3058430.21767079657556182No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC2783390.19809598992962174No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2541800.18090184530486655No Hit
AGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGA2479360.17645794286532138No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG2370070.16867969018085807No Hit
GTTTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTC2192880.15606894269105978No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT2028440.1443656224290674No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1893760.13478034407291845No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1808740.12872940580561976No Hit
CATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAG1779530.12665050781940718No Hit
GTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT1774550.12629607741984064No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1757990.12511748958513744No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1719880.12240517180853484No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1570590.11178008860546478No Hit
GGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAG1560100.11103350730196015No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTC1522120.10833044172454302No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1486980.10582950111394698No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1482860.1055362775705305No Hit
AATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCATA1465700.1043149872780482No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1462870.10411357401885676No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1440450.10251792551317768No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG1426630.10153434556899904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)