FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005741303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005741303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115862476
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTG4038910.34859517416147745No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAA3672410.31696284481267256No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC3343010.28853258754801686No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2525880.21800673412158048No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC2387950.20610210332463463No Hit
AGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGA2285560.1972649022277066No Hit
GGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAG2263320.19534538516162905No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG2262380.19526425449426782No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2103030.181510880192134No Hit
GTTTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTC2056100.17746038846951623No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1840420.1588452157711527No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1772180.1529554745576126No Hit
CATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAG1771290.15287865935128125No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1727130.1490672441697172No Hit
AATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCATA1692870.14611029027206357No Hit
GTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT1598070.13792817615946684No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1581270.13647818125343705No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1543740.1332389962044312No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1511260.13043567271944023No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTC1455460.125619618210127No Hit
GTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATC1407550.12148454345132413No Hit
GGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATC1381690.11925258700668541No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1343060.11591846181502284No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1321440.11405245646571543No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1280120.11048615947064691No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC1277780.11028419589444989No Hit
GCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAGC1254010.10823262572085893No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA1217930.10511858904171872No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATG1204370.10394823601042325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)