FastQCFastQC Report
Fri 27 May 2022
EGAF00005741313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005741313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145936654
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC5058490.3466223091561357No Hit
GTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTG4311640.295445995356314No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC3137700.21500424423873662No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAA2897820.1985669755043171No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2795590.1915618813625808No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2683110.1838544276888793No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG2682000.18377836729078356No Hit
AGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGA2413680.16539230781596514No Hit
AATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCATA2413170.1653573611465698No Hit
GTTTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTC2199840.15073937490714293No Hit
GGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAG2193720.15032001487439886No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT2131530.1460585768946025No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2117520.14509857132944817No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1996420.13680045042008432No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATG1901410.13029009148037615No Hit
CATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAG1877240.12863389344256174No Hit
GTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT1845120.12643293849946702No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC1806960.12381810535412167No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1787570.1224894466882871No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1719560.11782920554009686No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1702650.11667048361955729No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG1698690.11639913301013464No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1649690.1130415118329354No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1646310.11280990449458982No Hit
GCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAGC1518110.10402527112893789No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1512230.10362235658767399No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTC1506610.10323725799551359No Hit
GGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATC1499230.10273155913249868No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1492760.10228821609134604No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1491610.10220941477800362No Hit
ATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTC1485240.10177292402496771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)