FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005741388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005741388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25647455
Sequences flagged as poor quality0
Sequence length55
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGC2696021.0511842208125524No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACC1615790.6300001306172484No Hit
GGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAGCCTCGCCCTGG1388890.5415313137307386No Hit
CCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAG1341710.5231357263323009No Hit
GCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTA876430.3417220149133705No Hit
ACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC814710.3176572490330912No Hit
GGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAGCCTCGCCCTGGG607210.2367525354854897No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGG599860.23388675406585177No Hit
GCCGCGAGGGGGGTGCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCAGCCGC569470.2220376251756753No Hit
GGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGT539210.21023918357591428No Hit
GGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG527310.20559934699173857No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC471530.1838506003812074No Hit
GACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGT459390.17911718726088027No Hit
GCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCC453360.17676607679007528No Hit
CCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCA438260.17087855305721367No Hit
TGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGC433630.16907330571395876No Hit
TCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGT428370.1670224199633063No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTC424400.16547450809446784No Hit
TCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGG395190.15408546384036936No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTC394230.15371115769576357No Hit
GGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCC393130.1532822652384028No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC386050.15052175742193524No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC372270.14514890463790658No Hit
GGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAG368820.1438037419307296No Hit
GGCCGCGAGGGGGGTGCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCAGCCG368180.14355420450099238No Hit
CCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCAGAAGC364890.1422714261512497No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCC354380.13817355367228443No Hit
ACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCG352290.13735865800329897No Hit
TGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA343370.13388073007633702No Hit
AAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT339190.13225093873836605No Hit
CTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTA332870.1297867566197114No Hit
AATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTA319740.12466734028775954No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCC305740.11920870901225872No Hit
GCGGACGCGCGCGCGACGAGACGTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGC305000.1189201813591251No Hit
CGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCC293660.11449869002596945No Hit
GACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT282870.11029164492149415No Hit
CCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTT282330.11008109771515343No Hit
CAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG281500.10975747886096301No Hit
GCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATC281390.10971458961522693No Hit
GCGCCGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCG280230.10926230302382828No Hit
CGGACGCGCGCGCGACGAGACGTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCC261390.10191654493594005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)