FastQCFastQC Report
Fri 10 May 2024
EGAF00005741388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005741388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25647455
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length55
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAAC2745801.0705935540192975No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT1671020.6515344309990991No Hit
GGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAGCCTCGC1421340.5541836412228816No Hit
CCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGT1407640.5488419806175701No Hit
GCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGG923570.36010200622244976No Hit
ACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGC836430.32612592555479675No Hit
CCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCA737640.2876074838614592No Hit
GGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC720360.2808699732585553No Hit
GACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCG631210.24611018910063398No Hit
GGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAGCCTCGCC621140.24218387360461302No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAA612220.23870594567765108No Hit
CTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCT584600.22793684597555586No Hit
GCCGCGAGGGGGGTGCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCA584540.227913451841518No Hit
GGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCG561170.21880143663377127No Hit
GCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCC524800.20462069238448805No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCC496300.19350847871650423No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT484570.18893492551210245No Hit
TCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAG448010.17468009983836602No Hit
CCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGC446740.1741849240012313No Hit
TCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC441500.17214183629525814No Hit
TGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGC437730.17067190487321257No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGC403880.15747371425351953No Hit
GGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGC401890.15669780880793047No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG395600.1542453237562947No Hit
GGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATC384170.14978874122208227No Hit
GCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGC382030.14895435044139857No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCC381880.14889586510630393No Hit
GGCCGCGAGGGGGGTGCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCC381660.14881008661483175No Hit
AAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAA379830.14809656552667702No Hit
TCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTT372160.1451060153921705No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACC360590.14059484654520302No Hit
TGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCG360190.14043888565161727No Hit
ACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGC360030.140376501294183No Hit
GCGGACGCGCGCGCGACGAGACGTGGGGTGGGGGTGGGGGGGGGCGCGCC344400.13428232937732026No Hit
AATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGA332430.12961519963676707No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC314090.122464392665861No Hit
CGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCG310820.12118941236079758No Hit
CGGACGCGCGCGCGACGAGACGTGGGGTGGGGGTGGGGGGGGGCGCGCCG305630.11916581976652264No Hit
CAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCG291610.11369939044634253No Hit
CCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCC291120.11350833835170002No Hit
GACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAG290770.11337187256981247No Hit
GCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCG290410.11323150776558531No Hit
GCGCCGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCG284230.11082191195968566No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC262270.10225965890182866No Hit
CCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCAG261150.10182296839978859No Hit
CCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGGC257840.10053239200536661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)