FastQCFastQC Report
Sat 25 Jun 2022
EGAF00005741969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005741969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131890863
Sequences flagged as poor quality0
Sequence length100
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG15210511.1532648777952117No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC10920390.8279868484900277No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT6845880.5190564262211249No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG4916860.3727976213181652No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4337540.3288734262054226No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG3557710.26974651003686284No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA3208840.24329509467232768No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT3099810.23502841133126862No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT2954620.22402006725818452No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA2801990.21244762042386514No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG2761330.20936476850560906No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC2557010.19387317224544964No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG2270190.1721264042377219No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC2208130.16742099867827842No Hit
GCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC2009640.15237143455494714No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG1771160.1342898180899764No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG1762220.13361198493333082No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1552010.1176738073205268No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1471600.11157709992389692No Hit
ATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG1467740.11128443370637435No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC1425030.10804615024772413No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1375450.10428698157809461No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT1365330.1035196805103929No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC1341540.10171591643918503No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG1336310.101319376460521No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAG1331060.10092132007658483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)