FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005742372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005742372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22857291
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33250.036.74192445
CTCGTAT42700.028.55782144
CGTATCT45200.027.0778539
TATCTCG46900.026.33620641
GTTCCGT48400.025.28763435
TCCGTAT49050.025.04420937
GATCGGA52500.021.5769541
ACACGTC59550.020.93060113
GTATCTC65800.018.97670640
TCTCGTA70850.018.73552543
CACGTCT67650.018.5242714
CACACGT69700.018.01171912
CGTCTGA72950.017.36348216
TTCCGTA71400.017.20477936
TCGGAAG67700.016.9818883
AAGCGAC50350.016.1756920
CGCTATG51300.015.87617529
TCGCTAT51950.015.6341928
AAAAGCG52150.015.617372518
GAACGCT52450.015.22781235
Produced by FastQC (version 0.11.5)