FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005743524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005743524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18408702
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19100.010.82512448-49
CTCGTAT19350.010.80605644-45
CGTATGC19650.010.52254546-47
GATCGGA146550.010.41675394
AGATCGG166900.09.09035894
ATCGGAA136150.09.038959594
TAGATCG58350.08.69396294
TCGGAAG137500.08.26699194
TCGTATG20650.07.62351846-47
CGGAAGA145100.07.283663794
AAGATCG66150.06.88773994
ATCTCGT33300.06.20836442-43
GAGATCG83500.06.13161494
CAGATCG55350.05.68580594
ATGCCGT30950.05.542284550-51
TCTCGTA25900.05.44264144-45
TCCGATC15604.6709785E-55.4197936
ACACGTC60250.05.37931494
TGCCGTC45800.05.181822350-51
GCACACG92500.05.12878494
Produced by FastQC (version 0.11.5)