FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005743525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005743525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18408702
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA12650.013.75105946-47
GATCGGA143150.010.76497494
TAGATCG57250.09.60153994
AGATCGG164850.09.26242894
ATCGGAA133800.08.98906194
AGCGTCG56050.08.80124694
CCGTATC20850.08.57069148-49
GCCGTAT17300.08.56254248-49
GCGTCGT48700.08.39306894
TCGGAAG139950.08.09048694
TCGCCGT19350.07.896367546-47
GTCGCCG24950.07.628774644-45
CGTATCA19600.07.55817650-51
CGGAAGA142000.07.080368594
AGAGCGT86750.06.661408494
TGGTCGC27200.06.56371842-43
AAGATCG66300.06.377631794
GAGCGTC76050.06.36309694
GTCGTGT51150.06.24588194
CGTCGTG48200.06.237640492-93
Produced by FastQC (version 0.11.5)