FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005743538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005743538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16832600
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18100.010.774322548-49
TAGATCG49250.010.39551394
CTCGTAT18600.010.23154544-45
AGATCGG146500.09.9391694
GATCGGA128200.09.34281694
CGTATGC21800.08.51490546-47
TCGGAAG124400.08.34444694
ATCGGAA122900.08.21697994
AAGATCG58100.07.114394
CGGAAGA130400.06.807847594
TCGTATG19350.06.800114646-47
ATCTCGT32350.06.02759342-43
ACACGTC55050.05.9726494
TATCTCG22050.05.966499342-43
GAGATCG76550.05.70641594
TCTCGTA26200.05.55980544-45
CACGTCT65950.05.19916694
ATGCCGT31200.05.196907550-51
CAGATCG53450.05.096897694
CCGTCTT43550.05.018683452-53
Produced by FastQC (version 0.11.5)