FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005743583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005743583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48098275
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60800.015.00062946-47
CGTATGC63450.014.18751244-45
CTCGTAT66100.013.29765842-43
GATCGGA95600.011.794642
AGATCGG106900.010.3737561
ATCGGAA112500.010.1479613
ATCTCGT95200.09.38128940-41
CGTCTGA110300.08.47657116-17
TCGTATG71850.08.30894844-45
TCGGAAG149050.07.94316434
TGCCGTC121300.07.712625548-49
TCTCGTA78900.07.44677142-43
CACGTCT135500.06.96933714-15
AATCTCG90300.06.79310940-41
CGGAAGA178000.06.5984975
CCGTCTT145600.06.490256350-51
ATGCCGT98500.05.91827948-49
ACACGTC107100.05.637004414-15
CACACGT177850.05.61355812-13
GCCGTCT109200.05.575377550-51
Produced by FastQC (version 0.11.5)