FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99412948
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA520450.010.26818470
AGATCGG727000.09.7754470
GATCGGA597550.09.71155770
TAGATCG221600.09.33007370
TCCGATC89000.08.6103966
TTCCGAT97100.08.3245165
TCGTATG52600.07.384419445
TCGGAAG633050.07.04128470
AAGATCG360600.06.413882770
CGGAAGA621900.06.237776870
CCGATCT133150.06.2283817
CAGATCG357950.06.187247870
CGTATGC66300.05.54198546
CTCGTAT75800.05.355060644
Produced by FastQC (version 0.11.5)