FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52026329
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1101360.2116928142287341No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1003660.19291386097988963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA987950.1898942360511348No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG868560.1669462398548243No Hit
CTTCCATATATACATTCCTTGGAAAGCTGAACAAAATGAGTGAAAACTCTA808510.15540400707495622No Hit
CTTTTATACATGCATTCCTTGGAAAGCTGAACAAAATGAGTGAAAACTCTA525000.10091044478652339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA447600.025.26343345
CATGCAT268450.022.1596589
ATACATG289350.021.25876
AAACTCT548000.020.75777244
TGCATTC308100.019.78247511
TATATAC228650.019.6699497
AAAACTC588600.019.6402143
TACATGC313650.019.3534477
TTCCATA271650.019.2564092
ATATATA246150.018.6919736
CCATATA233250.018.6550164
TCCATAT278050.018.5056383
TTTATAC156700.018.4355833
ACATGCA327700.018.386458
CATATAT247300.017.8408665
TCATACA136250.017.8339714
TTATACA161600.017.5285174
ATATACA286300.017.2180828
CATACAT145600.016.5960775
TTTCATA153150.016.5565172
Produced by FastQC (version 0.11.5)