FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76711064
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA538500.010.34081294-95
ACACGTC790850.09.79517894-95
ACGTCTG657350.09.64699994-95
CACACGT951750.09.21098294-95
CACGTCT772450.09.20643294-95
GATCGGA2783150.09.0634894-95
ATCGGAA2516550.08.9994894-95
GCACACG1132750.08.906013594-95
AGATCGG3124400.08.74397994-95
TCGGAAG2416450.08.69331894-95
CGGAAGA2279450.08.52881194-95
TAGATCG890750.08.47404694-95
CAGATCG1161450.07.812646494-95
AGAGCAC1777650.07.79251294-95
AGCACAC1477450.07.681939694-95
AAGATCG919350.07.410324694-95
GAGCACA1713250.07.320657394-95
AAGAGCA2190150.07.073960394-95
GAAGAGC2438250.06.794898594-95
GAGATCG984950.06.747579694-95
Produced by FastQC (version 0.11.5)