FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54745433
Sequences flagged as poor quality0
Sequence length32-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA557250.10178931272678032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT41600.018.1181131
GTTCCTA137350.017.90384315
CCGGCTT193150.017.1232152
AACGCTA159050.017.08815423
AGTTACC144650.017.00175534
ACGCTAC160400.016.97247924
GCCGGCT174600.016.9063991
CCTACTC146800.016.73600218
TTCGACG30800.016.655196
CGCTACC166250.016.40239125
CCCGTAT37450.016.4010751
GTCCGAT28650.016.2557581
CTCGGTT112300.016.24811
TCACGCT179150.015.9003028
TTCACGC182100.015.8033467
AGACGCT251050.015.52420841
CGTTACG28750.015.41644821
CTCCGTT84950.015.228811
CCCCGCA242300.015.15515435
AAAACGC181600.015.07754521
Produced by FastQC (version 0.11.5)