FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54745433
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG699730.1278152279844056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG239050.015.51548445
GGGAGGT342700.014.76813
GGAGGTC337900.014.3179784
ATAGGGG267350.014.28168738
GGCATAG257750.014.2638535
GTCACTC350550.013.3570658
GAGGTCA373900.013.2879125
GAGCTTA285750.012.99491944
GGTCACT366200.012.7495347
ACTCTCC378900.012.58785711
TGGCATA293900.012.58620434
CATAGGG299300.012.40374137
AGGTCAC404700.011.987126
GCATAGG315200.011.80661936
TCACTCT416250.011.4433399
CACTCTC411850.011.23740210
TGGGAGG360550.010.9164822
TAGGGGA458150.010.57826139
GCTCTGT475450.010.17359822
AGGGGAG481200.010.141705540
Produced by FastQC (version 0.11.5)