FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123080812
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA394400.010.06705770
TCGCTAG54700.010.04361134
TCCGATC106750.09.7353956
AGATCGG493350.09.4614770
TAGATCG160000.09.11135270
ATCGGAA361400.08.63970870
TTCCGAT128250.08.34895
TCGTATG69850.08.06547945
CGCTAGA68900.07.92295135
CCGATCT161050.06.8006197
CGTATGC87400.06.56619946
TATGCCG96650.05.97400848
CTCGTAT93750.05.934674344
TCGGAAG494550.05.91678570
CTCGCTA114150.05.456611633
CAGATCG303000.05.216069770
CGATCTA59050.05.09615568
Produced by FastQC (version 0.11.5)