FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123080812
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA430900.09.264111570
CGCCGTA62800.09.19262658
AGATCGG530200.08.84994370
TAGATCG168150.08.22575270
ATCGGAA392250.08.09691870
CGTATCA76200.07.530162361
GCCGTAT76600.07.35381659
TCGGAAG586100.05.05445270
Produced by FastQC (version 0.11.5)