FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59769623
Sequences flagged as poor quality0
Sequence length34-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38750.022.1791676
ACCGCGT132100.020.2162916
GACCGCG133700.019.9578025
AGACCGC138950.019.430664
TCGACGA44600.019.3709727
CGACGAG42600.019.012888
CCGCGTT145100.018.436127
CGGTATA65300.018.123720
CGCGTTC150450.017.8104368
CAGACCG155800.017.5314223
AACGCTA165200.015.43102823
GTTCGAC53700.015.2505845
GAACGAT183500.015.17091745
GTTCCTA98650.015.02932215
ACGCTAC170050.014.97768924
GTCCGTT40350.014.6667451
CGCTACC175700.014.45762225
TCAGACC205900.014.26186852
CGTTACG31200.014.20648621
CCCGTAT37400.014.138971
Produced by FastQC (version 0.11.5)