FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59769623
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG610570.1021538984778271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG223150.013.77826545
ATAGGGG234650.013.41544938
GGCATAG236450.012.82752935
GGGAGGT348250.012.7809953
GGAGGTC337700.012.5139084
GAGCTTA260150.011.84427144
GTCACTC354200.011.4262048
CATAGGG271500.011.3707237
GGTCACT361550.011.24389657
ACTCTCC363450.011.07339411
TGGCATA280300.011.02129534
GCATAGG289100.010.63171836
TCAGACG92200.010.5436882
GTTGTCG32200.09.99170426
TCACTCT420850.09.8251149
GAGGTCA433250.09.8250485
CGTGGCG154750.09.8009671
TAGGGGA422800.09.77115239
AGGTCAC430650.09.7427476
CACTCTC410650.09.68559310
Produced by FastQC (version 0.11.5)