FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55096046
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1001010.1816845441141094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960010.17424299377127717No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC717620.1302489111469088No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG613650.11137822848485351No Hit
CTTCCATATATACATTCCTTGGAAAGCTGAACAAAATGAGTGAAAACTCTA608280.11040356689117038No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA596590.10828181753732383No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT596360.1082400722549128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2750.029.57576842
TAGTCCG2550.028.24629845
CTCGACG34950.027.27919242
TTCGCGT14100.026.85734443
CGGTACG5850.026.7678787
TGTAGCG13650.026.55124511
TAGCACG10800.025.6852116
CGCTAGC11800.025.40535510
TTTCGCG2650.025.3240813
TCTCGAC38300.024.80201541
AACTCTA298650.024.4588645
CATAGCG12700.024.30695522
GCGTTAG3600.023.01753437
TCGTATA3350.022.70325721
ATTCGCG7900.022.11504738
CGGACCG19950.021.8652671
TAAGCCG25150.021.7914663
GTAGCGA19100.021.78577412
ATAGCGC4650.021.64806623
CGCGATA1451.0913936E-1121.60043711
Produced by FastQC (version 0.11.5)