FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55650388
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA580270.10427061173409967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA242450.021.12449545
TATATAC134550.016.0718087
CATGCAT172800.015.9940749
ATATATA148550.015.4889096
CCATATA140750.015.2993624
AAACTCT351200.014.86479344
TTCCATA175050.014.57745552
CATATAT151600.014.3840015
ATACATG192500.014.2629766
TCCATAT177550.014.068563
ATATACA161450.013.8296958
TACATGC203150.013.47057157
TGCATTC215550.012.89101811
AAAACTC413400.012.78045743
TTATACA134350.012.0929124
TACATTC217450.011.77635711
TTTATAC134900.011.7726163
GCATTCC246400.011.36818912
ACATGCA248550.011.2200218
ATACATT202950.011.12473110
Produced by FastQC (version 0.11.5)